Research focus: (High Altitude Himalayan Medicinal Plants)

•         Development of plant cell and tissue culture systems for the sustainable production of secondary metabolites in Himalayan medicinal plants
•         Understanding the molecular cues linked with enhanced secondary metabolites in  cell culture
•         Aeroponic and Hydroponic farming for metabolite enriched plant biomass production and understanding the mechanism of nutrients towards growth and development. 
  

Publication last 5 years (from IHBT):

1.    Shiv Rattan, Dinesh Kumar, Ashish R Warghat* (2021) Growth kinetics, metabolite yield, and expression analysis of biosynthetic pathway genes in friable callus cell lines of Rhodiola imbricata (Edgew). Plant Cell, Tissue and Organ Culture, 146, 149–160, (IF-2.7).
2.    Kanika Thakur & Ashrita & Archit Sood & Pawan Kumar & Dinesh Kumar & Ashish R. Warghat* (2021) Steviol glycoside accumulation and expression profiling of biosynthetic pathway genes in elicited in vitro cultures of Stevia rebaudiana. In vitro cellular and Developmental Biology, Plant, 57: 214–224 (IF- 2.2).
3.    Pankaj Kumar, Ashrita, Vishal Acharya, Ashish R. Warghat* (2021) Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb, Phytochemistry, 183, 112631, (IF-4.0).
4.    Mahinder Partap, P Kumar, A Kumar, R Joshi, D Kumar, Ashish R Warghat* (2020) Effect of elicitors on morpho-physiological performance and metabolites enrichment in Valeriana jatamansi cultivated under aeroponic conditions. Frontier in Plant Science, 11, 01263 (IF-5.7).
5.    Mahinder Partap, P Kumar, Ashrita, P Kumar, D Kumar, Ashish R Warghat* (2020). Growth kinetics, metabolites production and expression profiling of picrosides biosynthetic pathway genes in friable callus culture of Picrorhiza kurroa Royle ex Benth. Applied Biochemistry and Biotechnology, 192, 1298–1317 (IF- 2.9).
6.    Shiv Rattan, Archit Sood, Pankaj Kumar, Anil Kumar, Dinesh Kumar, Ashish R Warghat* (2020). Phenylethanoids, Phenylpropanoids, and Phenolic acids quantification vis-a-vis gene expression profiling in leaf and root derived callus lines of Rhodiola imbricata. Industrial Crop and Products, 154,112708, (IF-5.6).
7.    Pankaj Kumar, Mahinder Partap, Ashrita, Divya Rana, Pawan Kumar, Ashish R. Warghat* (2020). Metabolite and expression profiling of steroidal alkaloids in wild tissues compared to bulb derived in vitro cultures of Fritillaria roylei - high value critically endangered Himalayan medicinal herb, Industrial Crops and Products, 145, 11945 (IF-5.6).
8.    Kanika Thakur, Mahinder Partap, Dinesh Kumar, Ashish R Warghat* (2019). Enhancement of picrosides content in Picrorhiza kurroa Royle ex Benth. mediated through nutrient feeding approach under aeroponic and hydroponic system. Industrial Crops and Products, 133, 160-167 (IF-5.6).

Books/Chapters

1. Pankaj Kumar, Ashrita, Mahinder Partap and Ashish R. Warghat* (2021) Fritillaria roylei; Himalayan Medicinal Plants, Advances in Botany, Production & Research Elsevier, ISBN: 9780128231517, pp. 57-64.

2. Pushpender Bhardwaj, Shiv Rattan, Avilekh Naryal, Ashwani Bhardwaj and Ashish R. Warghat* (2021) Valeriana jatamansi, Himalayan Medicinal Plants, Advances in Botany, Production & Research Elsevier, ISBN: 9780128231517, pp. 259-268.

Citations: https://scholar.google.co.in/citations?user=5YaCUhcAAAAJ&hl=en

Reviewer

AWSAR (DST, New Delhi)

Editorial Board Member:

BMC Plant Biology and BMC Biotechnology

Awards/recognition

1. Shiv rattan, Ashish R. Warghat* got 2nd prize in poster presentation Entitled “In vitro propagation of Rhodiola imbricata (Edgew.): a high value rare medicinal herb” in International conference on conservation and sustainable use of high altitude medicinal and aromatic plants for the socio-economic development, Dehradun (29th-30th Nov., 2020).

2. Kanika Thakur, Ashish R Warghat* awarded as Best research scholar award for the paper presented entitled “Enhancement of picrosides content in Picrorhiza kurroa Royle ex Benth. mediated through nutrient feeding approach under aeroponic and hydroponic system” in Recent advances in Agricultural, environmental and applied Sciences for Global development, Nauni University, Solan (27th-29th Sept., 2019).

3. Research Associate ship, DIHAR (DRDO), Chandigarh, 2014

4. Travel grant received from Department of Biotechnology (DBT) and Human Resource Development Group, Council of Scientific and Industrial Research (CSIR) to attend World Orchid Conference, at Singapore, 2011.

5. JRF and SRF- DIHAR (DRDO), Leh, 2009 and 2011.

 MOU Signed:

1. Amar Exports, Hyderabad-Tissue culture protocol & hydroponic cultivation of P. kurroa and V. jatamansi

2. HYDROCROPS INDIA PVT LTD, Jalandhar-Cultivation and production of Kutki and Jatamansi plants under hydroponic system 

3. Sh. Akshat Goel, New Delhi-Establishment of hydroponic facility for production of Lilium and Tulip plants

Nil

Interested and highly motivated students with independent fellowship like CSIR/UGC/DBT/INSPIRE are welcome to contact for PhD. Also candidates seeking for post doctoral research are encouraged to write their own research proposals for suitable funding of agencies like DST,CSIR,DBT etc.  

Research Team:

1. Mr. Shiv Rattan, Ph. D. Scholar (UGC-JRF), Topic: Enhancement of phenyl alcohol glycosides in cell culture of Rhodiola imbricata (Edgew.) 

2. Mr. Mahinder Partap, Ph. D. Scholar (CSIR-JRF), Topic: Plant cell culture for iridoid glycosides production in Picrorhiza kurroa Royle ex Benth.

3. Ms. Ashrita, Ph. D. Scholar (DBT-JRF), Topic: Plant cell culture for the enhancement of rebaudioside content in Stevia rebaudiana

4. Ms. Kanika Thakur, Ph. D. Scholar, Topic: Hydroponic and aeroponic farming for enhancement of picrosides content in Picrorhiza kurroa 

 Start-up: 

1. Sanjeev Kumar (Sanjeev Kumar Nursery, Kullu)-Cultivation of High Value lower Crops (Lilium and Tulip) using Hydroponic system

2. Kanik Kaushal (M/S Yuktika Biotech and Nutraceuticals Pvt. Ltd., Palampur)-Hydroponic Production of Strawberry and Cardamom

Past researchers:

Dr. Archit Sood (Currently working as Post Doc, at Institute of Plant Sciences, Volcani Center, Agricultural Research Organization (ARO), Rishon Lezion, Israel)

Dr. Pankaj Kumar (Currently working as Assistant Professor at Dr. Y. S. Parmar University of Horticulture & Forestry, Solan, HP, India)