Biotechnology
Senior Principal Scientist
Plant tissue culture, Development of transgenic crops for abiotic stress tolerance
01894-233339 Ext 394
Ph.D.

Research Focus: 

Major focus of the group is conservation and improvement of commercially important plants of western Himalaya. Therefore, studies are being undertaken to gain basic understanding of the biology of medicinal plants, saffron, bamboo and ferns using transcriptome, proteome biochemical and in vitro approaches with an aim to:

•Develop in vitro systems for plant regeneration.

•Produce and modulate secondary metabolites in alternative systems. 

•Genetically modify and develop transgenic crop plants for abiotic stress tolerance.

•Mass multiply and rehabilitate high value endangered/critically endangered medicinal plants of mainly, alpine/sub-alpine Himalayas like Fritillaria roylei, Nardostachys jatamansi, Picrorhiza kurroa, Dactylorrhiza hatagirea for their conservation and sustainable utilization.

 

In addition, micropropagation and macropropagation of commercially important plants like potato, saffron, apple scion and rootstock, bamboos, lavender, large cardamom, coleus, scented rose, ferns etc. are catering to the demands of the industry and farmers. 

Other major activities of the group include incubation of new entrepreneurs and training of young graduates/post-graduate students in plant tissue culture in order to increase their employability.

 

Awards:  

•National Merit Scholarship for second position in B.Sc. (Hons.)

•China Chinese Government Scholarship (1994-1996) under bilateral exchange program, Human Resources Development, Govt. of India

•Raman Research Fellowship (2005 to 2006), CSIR.

Publications:  A total of 70 publications; 1279 Citations: H index: 15; i10 index: 23

 

A few significant publications in the area of:

Transgenic crops (Tea)

1.Sandal I, Koul R, Saini U, Mehta M, Dhiman N, Kumar N, Ahuja PS, Bhattacharya A (2015) Development of transgenic tea plants from leaf explants by the biolistic gun method and their evaluation. Plant Cell Tiss Organ Cult.  DOI 10.1007/s11240-015-0828-x.

 

2.Bhattacharya A., Sharma M., Gulati A., Joshi R., Chanda S.K., Ahuja P.S. (2014) Histochemical evaluation of catechins in PEG stressed transgenic tea plants by catechin-specific-diazotized sulfanilamide reagent. Biotechnic & Histochemistry. 90(1):45-54, DOI: 10.3109/10520295.2014.942369 

 

3.Bhattacharya A., Saini U., Joshi R., Kaur D., Pal A.K., Kumar N., Gulati A., Mohanpuria P., Yadav S.K., Kumar S., and Ahuja P.S. (2014) Osmotin expressing transgenic tea plants have improved stress tolerance and quality parameters. Transgenic Research 23(2): 211-223, DOI: 10.1007/s11248-013-9740-5. 

 

4.Kumar N., Bhattacharya A., Gulati A. (2013) L-glutamine and L-glutamic acid facilitate successful Agrobacterium infection of recalcitrant tea cultivars. Applied Biochemistry and Biotechnology. DOI 10.1007/s12010-013-0286-z. 

 

5.Saini, U. Kaur. D, Bhattacharya A., Kumar, S., Singh, R. D., Ahuja P. S. (2012) Optimising parameters for biolistic gun-mediated genetic transformation of tea [Camellia sinensis (L.) O. Kuntze]. The Journal of Horticultural Science & Biotechnology 87 (6): 605-612.

 

6.Sandal I, Bhattacharya A., Saini U, Kaur D, Sharma S, Gulati A, Kumar, JKK, Kumar N, Dayma J, Das, P, Singh B, Ahuja PS (2011) Chemical modification of L-glutamine to alpha-amino glutarimide on autoclaving facilitates Agrobacterium infection of host and non-host plants: A new use of a known compound. BMC Journal of Chemical Biology 11:1-13. DOI:10.1186/1472-6769-11-1.

 

7.Bhattacharya A., Saini U., Sharma P., Nagar P.K. and Ahuja P. S. (2006) Osmotin-regulated reserve accumulation and germination in genetically transformed tea somatic embryos: A step towards regulation of stress tolerance and seed recalcitrance. Seed Science Research 16: 203-211. 

 

8.Sandal I., Saini U., Bhattacharya A., Lacroix B., Kumar S., Ahuja P. S. and Citovsky V. (2006) Agrobacterium-mediated genetic transformation of tea leaf explants: Effects of counteracting bactericidity of leaf polyphenols without loss of bacterial virulence. Plant Cell Reports. 26(2): 169-176. 

 

9.Mondal T. K., Bhattacharya A., Ahuja P. S., Chand, P. K. (2001) Transgenic tea (Camellia sinensis  (L..) O. Kuntze cv. Kangra jat) plants obtained by Agrobacterium mediated transformation of somatic embryos. Plant Cell Reports 20(8): 712-720. 

Endangered plants of Himalayas:

1.Patial V, Devi K, Sharma M, Bhattacharya A., Ahuja PS (2012) Propagation of Picrorhiza kurrooa Royle ex Benth.: An important medicinal plant of western Himalaya. Journal of Medicinal Plant Research 6(34): 4848-4860; DOI: 10.5897/jmpr12.115.

 

2.Kushwaha, R., Bhattacharya A., Singh B., Singh R.D. (2012) Factors affecting podophyllotoxin yield in the ex situ grown plants of Podophyllum hexandrum, an endangered alpine native of western Himalaya. Journal of Natural Medicine. 66: 1-7; DOI 10.1007/s11418-011-0541-y. 

 

3.Kushwaha R., Chanda S, Ogra R.K. Bhattacharya A (2010) Cryopreservation criteria of Podophyllum hexandrum and Aconitum heterophyllum seeds based on storage behavior. Seed Technology 32 (2) 117-127

 

4.Kushwaha R., Pandey S., Chanda S., Bhattacharya A., Ahuja P. S. (2008) Temperature dependant growth and emergence of functional leaves: an adaptive mechanism in the seedlings of the western Himalayan plant Podophyllum hexandrum syn. P. emodi. Journal of Plant Research 121(3):299-309. 

 

5.Kharkwal A., Kushwaha R., Prakash O., Ogra R.K., Bhattacharya A., Nagar P.K. and Ahuja P. S. (2008) An efficient method of propagation of Podophyllum hexandrum: an endangered medicinal plant of Western Himalayas under ex situ conditions. Journal of Natural Medicine 62(2): 211-216. 

 

6.Kushwaha R., Pandey S., Chanda S., Bhattacharya A., and Ahuja PS. (2007) GA3 induced changes in the slow growing endangered Himalayan plant Podophyllum hexandrum and hastening of vegetative growth. Plant Growth Regulation 51(3): 207-215.

Bamboo

1.Kaur D., Dogra V., Thapa P., Bhattacharya A., Sood A., Sreenivasulu Y. (2015) In vitro flowering associated protein changes in Dendrocalamus hamiltonii. Proteomics 15: 1291-1306. 

2.Kaur D, Thapa P, Sharma M, Bhattacharya A., Sood A (2014) In vitro flowering – A system for tracking floral organ development in Dendrocalamus hamiltonii Nees et Arn. ex Munro. Indian Journal of Experimental Biology. 52: 825-834.

 

3.Sood. P., Joshi R., Chanda S., Bhattacharya A., Gulati A., Sood A. (2013) A method to overcome the waxy surface, cell wall thickening and polyphenol induced necrosis at wound sites - the major deterrents to Agrobacterium mediated transforma

•A total of 08 Patents granted including 06 from US.

The research group:

 

Students

 

Bhuvnesh Sareen (ICMR Senior Research Fellow) is trying to understand the gametophyte resources of ferns and mechanisms underlying its biology.

Nisha Dhiman (UGC NET Senior Research Fellow)

Neetu Gautam (ICMR Senior Research Fellow) is developing alternative systems for secondary metabolite production in saffron.

Isha Sharma (PA level II) and Priya Brat Shethi (PA level I) are attempting micropropagation of endangered and critically endangered medicinal plants of Himalayas.  

Ishu Sharma (PA level II) is developing micropropagation systems of commercially important bamboos for industrial applications.

 

Technical Staff

 

Shri Om Prakash (Principal Technical Officer) is working on bamboos, medicinal plants of alpine/sub-alpine Himalaya, commercially important plants like tea, saffron, seabuckthorn, Gingko biloba, ginseng etc. wrt R&D, their promotion and commercialization.

Dr. Kiran Devi (Senior Technical Officer) is working on tissue culture of commercially important plants including alpine/sub-alpine plants of western Himalaya. 

Parveen Sharma (Senior Technician) and Shri Ajay Kumar (Field Assistant) are catering to industrial and societal demands through mass multiplied plants of commercial importance. 

 

Powered by ChronoForms - ChronoEngine.com