Please visit: https://sites.google.com/site/drdineshkumarcsirihbt/

Research Focus

From the primitive age, plants and marines have been used to cure serious complications of health and are still very significant. Their derived products are used either as such or template in the discovery of new drugs. Several biologically active secondary metabolites have been used for their medicinal values. Hence to understand the chemistry, metabolite composition and alterations in plant, marines and biofluids, along with quality profile and quality control, is the need of hours. We are currently working on metabolite profiling, and quality control of some medicinally important crops of western Himalaya using NMR, UPLC-MS/MS and HPTLC techniques. Moreover, we are also working to find out mechanistic role of medicinal plants and their derived products (Lower to Trans Himalaya) using modern analytical techniques based metabolomic approaches for toxicity, safety and action in health disorders such as Brain, cancer, diabetes, cardiovascular, inflammatory, respiratory, liver disorders.    

Major Research Interests  

• Isolation and characterization of secondary metabolites from nature.
• Process development for the isolation of industrially important chemicals.
• Development and validation of quality control methods.
• NMR based chemical signatures for metabolomics and quality control.
• Metabolomics studies to understand the chemistry, metabolite formation and their up and down regulations in respect to biotic and abiotic factors.
• Pharmacognostical studies, standardization and evaluation of traditional medicines.
• Herbal and Ayurvedic product development including NDDS.

External Funded Projects                                                                                                                  

Technology transferred: 11 product transferred to more than 15 Technology partners as PI and Co-PI

Varieties developed: Developed as Co-PI by contributing through chemical analysis and variability studies. (PGRC: Plant germplasm registration committee-ICAR-NBPGR Delhi)

Germplasm Registered at NBPGR

1. Germplasm CSIR-IHBT-ST-03 of Stevia rebaudiana (tetraploid) IC0635703, INGR20095 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 21st December, 2020
2. Germplasm CSIR-IHBT-TM-09 of Tagetes minuta (wild marigold) IC0630603, INGR20103 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 21st December, 2020
3. Germplasm CSIR-IHBT-TM-03 of Tagetes minuta (wild marigold) IC0630602, INGR20104 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 21st December, 2020
4. Germplasm CSIR-IHBT-VJ-05 of Valeriana jatamansi (Indian valerian / Tagar) IC0630604, INGR20096 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 21st December, 2020
5. Germplasm CSIR-IHBT-RD-04 of Damask rose IC0635435, INGR20105 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 21st December, 2020
6. Germplasm CSIR-IHBT-VJ-08 of Valeriana jatamansi (Indian valerian) IC0630605, INGR20057 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 29th September, 2020
7. Germplasm CSIR-IHBT-AM-02 of Artemisia maritima (Seaworm wood) IC0635705, INGR20060 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 29th September, 2020
8. Germplasm CSIR-IHBT-DH-04 of Dracocephalum heterophyllum (white dragonhead) IC0635704, INGR20059 has been registered by Plant Germplasm Registration Committee (PGRC) of ICAR on 29th September, 2020
9. Germplasm CSIR-IHBT-DH-04 of German Chammomill (INGR24046), 22 May, 2024.
10. Germplasm CSIR-IHBT-DH-04 of True Lavender (INGR24047), 22 May, 2024

Publications (2022-2023)

1.  Bhattacharyya P, Kumar S, Lalthafamkimi L, Sharma R, Kumar D, Singh D, Kumar S. Molecular and phytomedicinal stability of long term micropropagated Malaxis acuminata: An endangered terrestrial orchid of biopharmaceutical importance. South African Journal of Botany. 2023 Apr 1;155: 372-382. IF: 3.31
2.  Kumar R, Kumar D*. Comprehensive metabolomics and antioxidant activity of Allium species viz Allium semenovii, A. sativum and A. cepa: an important spice. Food Research International. 2023: 112584. IF: 7.425.
3. Walia S, Kumar P, Kumar D*, Kumar R*. A preliminary study on suitability of growing ginseng (Panax ginseng Meyer) in the Western Himalayan region. Plant Soil Environ. 2023;69. IF: 2.328
4. Kumari V, Kumar D, Bhardwaj R, Kumar D*. Metabolome analysis, nutrient and antioxidant potential of aerial and underground parts of Ajuga parviflora Benth. Microchemical Journal. 2023: 108451. IF: 5.304
5. Sood A, Thakur K, Joshi R, Kumar D, Warghat AR. Comparative quantification of dactylorhin among different wild tissues and in vitro cultures of Dactylorhiza hatagirea (D. Don), an endangered medicinal orchid of Western Himalayas. South African Journal of Botany. 2023; 153: 172-7. IF: 3.31
6. Shakeya P, Thakur R, Sharan H, Yadav N, Kumar M, Chauhan R, Kumar D, Kumar A, Singh S, Singh S*. GE biplot and regression based multi-environment investigations for higher yield and essential oil content in German chamomile (Matricaria chamomilla L.). Industrial Crops & Products. 2023, 193: 116145. IF: 6.449
7. Kumar D, Kumari V, Kumar D*. Metabolite profiling, antidiabetic, and antioxidant potential of different tissues of Trillium govanianum Wall. ex D. Don. South African Journal of Botany. 2023: 23: 102-108. IF: 3.31
8. Rattan S, Kumar D*, Warghat AR. The influence of phenylalanine feeding on cell growth, antioxidant activity, phenylpropanoids content, and yield in cell suspension culture of Rhodiola imbricata (Edgew.). Plant Cell, Tissue and Organ Culture (PCTOC). 2022 Nov;151(2):347-59. IF: 2.726
9. Devi J, Kumar A, Kumar D, Bhushan S. Adventitious root cultures of Arnebia euchroma: A sustainable alternative for the production of natural pigments. Industrial Crops and Products. 2022 Nov 1;187: 115461. IF: 6.449
10. Kumari V, Joshi R, Chawla A, Kumar D*. Metabolome analysis of Dactylorhiza hatagirea (D. Don) Soo reveals a significant antioxidant and nutritional potential of its tubers. South African Journal of Botany. 2022 Nov 1; 150:431-442. IF:3.31
11. Partap M, Rattan S, Kumar D*, Warghat AR. Bioprocess development for enhancing cell biomass, differential picrosides accumulation, and gene expression profiling at shake flask levels in suspension cultures of Picrorhiza kurroa. Industrial Crops and Products. 2022 Nov 1;187: 115311. IF: 6.449
12. Bhattacharyya P, Lalthafamkimi L, Sharma T, Kumar D, Kumar A, Kumar S. Metabolic and tissue-specific expression profiling in micropropagated plants of Malaxis acuminata: An endangered medicinal orchid. Plant Cell, Tissue and Organ Culture (PCTOC). 2022 Oct 7:1-5. IF: 2.726
13. Yadav N, Shakya P, Kumar A, Gautam RD, Chauhan R, Kumar D, Kumar A, Singh S, Singh S. Investigation on pollination approaches, reproductive biology and essential oil variation during floral development in German chamomile (Matricaria chamomilla L.). Scientific Reports. 2022;12(1):1-3. IF: 4.996
14. Partap M, Kumar P, Kumar P, Kumar D*, Warghat AR. Growth kinetics, metabolites production and expression profiling of picrosides biosynthetic pathway genes in friable callus culture of Picrorhiza kurroa Royle ex Benth. Applied Biochemistry and Biotechnology. 2020 Dec;192(4):1298-317. IF: 3.094
15. Sharma R, Kumar D*. Polyphenol Encapsulates from the Waste Biomass of C. sinensis and Their Simulated Oral, Gastric, and Intestinal Stability Studies: Nutraceutical Application. ACS Food Sci. Technol. 2022, 2, 8, 1257–1265. IF:
16. Kumari A, Kumar D*, Gulati A, Maurya SK. Mechanistic studies on polyphenol rich fractions of Kangra tea by HPTLC and NMR for their antioxidant activities. Journal of Food Science and Technology. 2022:59 (7): 2751-2763. IF: 3.117
17. Kaundal R, Kumar M, Kumar S, Singh D, Kumar D*. Polyphenolic Profiling, Antioxidant, and Antimicrobial Activities Revealed the Quality and Adaptive Behavior of Viola Species, a Dietary Spice in the Himalayas. Molecules. 2022 Jan;27(12): 3867. IF: 4.927
18. Partap M, Chhimwal J, Kumar P, Kumar D*, Padwad Y, Warghat AR. Growth dynamics and differential accumulation of picrosides and its precursor metabolites in callus cell lines of Picrorhiza kurroa with distinct anti-steatotic potential. Process Biochemistry. 2022 Jun 6. IF: 4.885
19. Sinha K, Kumar S, Rawat B, Singh R, Purohit R, Kumar D*, Padwad Y. Kutkin, Iridoid glycosides enriched fraction of Picrorrhiza kurroa promotes insulin sensitivity and enhances glucose uptake by activating PI3K/Akt signaling in 3T3-L1 adipocytes. Phytomedicine. 2022 May 25:154204. IF: 6.656
20. Partap M, Kumar P, Kumar P, Pal PK, Kumar D, Warghat AR, Kumar S. Fruit derived callus and cell suspension culture as promising alternative sources for mogrosides production in Siraitia grosvenorii (Swingle) C. Jeffrey: a zero-caloric natural sweetener. Journal of Food Composition and Analysis. 2022 May 1; 108: 104450. IF: 4.52
21. Joshi R, Sharma S, Kumar D*. Advances of Ion Mobility Platform for Plant Metabolomics. Critical Reviews in Analytical Chemistry. 2022 Apr 26:1-7. IF: 5.686
22. Kumar A, Joshi R, Vasishth A, Guleria V, Kumar D*. UHPLC-QTOF-IMS-Based Metabolite Fingerprinting of Underutilized Cordia myxa Fruits and Leaves: A Nutraceutical Source. ACS Food Science & Technology. 2022 Apr 20;2(5):793-807. IF:
23. Sharma A, Tirpude NV, Bhardwaj N, Kumar D, Padwad Y. Berberis lycium fruit extract and its phytoconstituents berberine and rutin mitigate collagen–CFA-induced arthritis (CIA) via improving GSK3β/STAT/Akt/MAPKs/NF-κB signaling axis mediated oxi-inflammation and joint articular damage in murine model. Inflammopharmacology. 2022 Apr;30(2):655-66. IF: 5.093

Patents

Ph. D Research Scholar

Doctoral students supervised – completed & on-going

Supervised other than Ph.D:  MD: 11

Co-Superviser of MD student of Rajiv Gandhi Govt. Post Graduate Ayurvedic College Paprola, H.P. (India)

Training imparted (M.Pharm, MSc students)

• Ms. Ankita Rani (2024) Extraction, fractionation, isolation of molecules from natural matrices along with chromatography (UPLC-PDA) and In-vitro biological assays From Central University of Haryana Jant-Pali, Mahendergarh, Haryana Supervised by Dr. Dinesh Kumar (25-02-202025 to 24-03-2025).
• Ms. Nikita (2024) Extraction, fractionation, isolation of molecules from natural matrices along with chromatography (UPLC-PDA) and In-vitro biological assays From Central University of Haryana Jant-Pali, Mahendergarh, Haryana Supervised by Dr. Dinesh Kumar (25-02-202025 to 24-03-2025).
• Mr. Priyanshu (2024) Experiential learning on Analytical techniques (UPLC-PDA, LC-MS/MS), Column chromatography, Phyto-nanosponge, and herbal soap From Department of Chemical Engineering, National Institute of Technology, Hamirpur, Himachal Pradesh Supervised by Dr. Dinesh Kumar (13-05-202024 to 26-06-2024).
• Ms. Ayushi Walia (2024) Hands-on training on column chromatography and analytical methods used in natural product research From Ward No. 02, Village Kir chamba, P.O. Hatwas, Tehsil Nagrota Bagwan, District Kangra, Himachal Pradesh Supervised by Dr. Dinesh Kumar (05-04-202024 to 29-09-2024).
• Ms. Neha Thakur (2024) Phytochemical & Therapeutic potentials of Urtica dioica from Department of Pharmacy, School of Pharmaceutical and Health Sciences, Career Point University, Hamirpur, Himachal Pradesh Supervised by Dr. Dinesh Kumar (1-07-202023 to 30-06-2024).
• Ms. Avni Sood, (2024) Hands on training for chromatography (column chromatography and Ultra-performance liquid chromatography) From School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi Supervised by Dr. Dinesh Kumar (10-06-202024 to 09-07-2024).
• Ms. Anshika, (2024) Hands on training for chromatography (column chromatography and Ultra-performance liquid chromatography) From Khalsa College of Pharmacy & Technology, Amritsar, Punjab Supervised by Dr. Dinesh Kumar (07-06-202024 to 06-07-2024).
• Miss Aman Patial, Preparation and characterization of phytosomal-phospholipid complex of bioactive fraction of Polygonatum verticillatum. Sri Sai College of Pharmacy, Pathankot, Punjab Technical University, Jalandhar (Punjab), 2016
• Miss Malvika Rana, Preparation and characterization of phytosomal-phospolipid complex of bioactive fraction of zanthoxylum armatum, Sri Sai College of Pharmacy, Pathankot, Punjab Technical University, Jalandhar (Punjab), 2016
• Ms.  Kirti, Chemical and biological investigation of Picrorhiza kurroa leaves found in western Himalaya. Faculty of Pharmaceutical Studies, Uttarakhand Technical University, (UK), 2016-2017.
• Nittya K. Dogra, Chemical Prospection of Vernonia species, Punjabi University Patiala, India. April 2017-Nov.2017.
• Miss. Sheetal Sharma, Extraction and sample preparation for analytical techniques, GC-MS, FTIR spectroscopy, Total phenolics, flavanoids and antioxidant assay. Junior Research Fellow, GBPNIHESD, Himachal Unit, Mohal-Kullu (HP) 175126, 2017
• Miss. Monika, Isolation of secondary metabolites using different chromatographic techniques. June– July 2017. Amity University, India.
• Miss. Komal, Hands on chromatographic techniques, Amity University, Haryana, 2017
• Miss. Diksha, Extraction, fractionation and isolation of bioactive molecules from Plant matrix (Tea). Dolphin PG College of Science & Agriculture, (Pb), 2017
• Miss. Diksha Dogra, Chemical Investigation of Sea Buckthorn (Hippophae rhamnoides) Berries using Chromatographic Techniques. Department of Chemistry, Amity School of Applied Sciences Gurgaon, 2016
• Miss Svati Kaushal, Chemical fingerprinting of Camellia sinensis cultivated in North-Western Himalayas. NIT Jalandhar, 2014
• Miss Swati, Chemical fingerprinting of Stevia rebaudiana. 2014

Project Fellow

Mr. Sachin Vashisath, Iva, Sweety

 Prospective Team Members: Shiv Kumar, Virat Abhishek, Pawan Kumar, Vijaylata 

 Postdoctorate  and Ph.D positions are available for self-motivated persons.