Biotechnology
Senior Scientist
Microbiology and Molecular Biology
01894-233339 Ext. 422
Ph.D.

Bioprospecting genes from higher altitude microbes/plants. Extreme climatic conditions and topography of higher altitude affect both microbes and plants. In particular, we are interested in higher altitude microbes/plants functional genomics with the major focus on enzymes of industrial relevance. Currently, we are targeting therapeutic proteins and industrially relevant enzymes from higher altitude microbes. Our strategy includes multi-dimensional approaches such as whole genome sequencing, proteomics, genetics, and bioinformatics to develop a bioprocess for the industrial use.

Small RNA-mediated gene regulation in bacteria. Small RNA regulators in bacteria are groups of small non-coding RNAs (sRNAs) ranging from 50 to 500 nts that are involved in gene regulation at the post-transcriptional level. sRNAs function to adjust bacterial physiology in response to different environmental cues. Therefore, we are interested in studying the role of sRNAs in understanding adaptation of higher altitude microbes in response to growth, stress, and changes in environmental conditions.

Selected publications: 

1.       Singh RM., Singh D*., Hallan V. (2017). Movement protein of Apple chlorotic leaf spot virus is genetically unstable and negatively regulated by Ribonuclease E in E. coli. Scientific Reports| 7: 2133 | DOI:10.1038/s41598-017-02375-y (*shared corresponding authorship).

2.       Singh S., Singh D., Kumar S. (2017). Expression and biochemical analysis of codon-optimized polyphenol oxidase from Camellia sinensis (L.) O. Kuntze in E. coliProcess Biochemistry https://doi.org/10.1016/ j.procbio.2017.05.008.

3.       Kumari A*., Singh D*., Kumar S. (2016). Biotechnological interventions for harnessing podophyllotoxin from plant and fungal species: Current status, challenges, and opportunities for its commercialization. Accepted for publication in Critical Reviews in Biotechnology: http://dx.doi.org/10.1080/ 07388551.2016.1228597(*shared first and corresponding authorship).

4.       Kumar A., Sharma M., Bharadwaj PK., Vats SK., Singh D., Kumar S. (2016). Copper, zinc superoxide dismutase from Caragana jubata: A thermostable enzyme that functions under a broad pH and temperature window. Process Biochemistry 51(10): 1434–1444.

5.       Kumar R*., Singh D*., Swarnkar MK., Singh AK and Kumar S. (2016). Complete genome sequence of Arthrobacter alpinus ERGS4:06, a yellow pigmented bacterium tolerant to cold and radiations isolated from Sikkim Himalaya.  Journal of Biotechnology220 (2016) 86–87(*Corresponding author).

6.       Himanshu, Swarnkar MK., Singh D., Kumar R. (2016).  First complete genome sequence of a species in the genus Microterricola, an extremophilic cold active enzyme producing bacterial strain ERGS5:02 isolated from Sikkim Himalaya Journal of Biotechnology 222: 17–18.

7.       Kumar R*., Singh D*., Swarnkar MK., Singh AK and Kumar S. (2015). Complete genome sequence of Arthrobacter sp. ERGS1:01, a putative novel bacterium with prospective cold active industrial enzymes, isolated from East Rathong glacier in India. Journal of Biotechnology 214:139-140 (*shared first authorship).

8.       Kaberdin VR*, Singh D* and Lin-Chao, S. (2011). Composition and conservation of the mRNA-degrading machinery in bacteria. J Biomed Sci 22: 18:23 (*shared first authorship).

9.       Singh D., Chang S.J., Lin P-H., Averina OV, Kaberdin VR and Lin-Chao, S. (2009). Regulation of ribonuclease E activity by the L4 ribosomal protein of Escherichia coli. Proc. Natl. Acad. Sci. USA 106: 864-869.

10.    Lin P-H., Singh D, Bernstein. J.A., and Lin-Chao, S. (2008). Genomic analysis of mRNA decay in E. coli using DNA microarrays. Methods Enzymol447: 47-64.

 

 · Selected “Assistant Director” State Forensic Science Laboratory, Junga, Shimla, Himachal Pradesh through Public Service Commission (open competition) in the year 2009.

  · Awarded “Distinguished Postdoctoral Fellowship” by Academia Sinica, Taiwan for year 2009-2011.

 ·Awarded “Young Scientist International Travelling Fellowship” by Japan Society for Promotion of Science (JSPS, Japan) for participation in the JSPS-DST Asian Academic Seminar 2008 held at Jawarharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.

 ·Awarded “International Travelling Fellowship” by Institute of Molecular Biology, Academia Sinica, Taiwan for the year 2007.

 ·Selected as Scientist Gr. IV(1) at CSIR-NEIST, Jorhat, India (2005).

 

Post-doctoral Fellows:    Dr. Vijay Kumar, Dr. Virender Kumar, Dr. Prakriti Kashyap 

PhD Fellows: Sanyukta Darnal, Ambika, Subhash Kumar

Project Fellows: Vikas Thakur, Vijeta Patial, Anju Yadav, and Deepika Naag

 

 

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