Biotechnology
Principal Scientist
Molecular Genetics & Genomics
01894-233339 Ext. 358,458
,
Ph.D.

Our current efforts are to exploit NextGen Sequencing technologies for identification genome wide molecular markers and high throughput genotyping for dissection of complex traits, plant diversity evaluation and genome mapping in tea, stevia and targeted plant species of north western Himalayan region.

Fellowships/ Associateships/ Awards:

     ·         Indo-US Science & Technology Research Fellowship Award ( 2010)

Dr Edward S Buckler’s Maize Genetics Group, Institute of Genomic Diversity, Cornell University, Ithaca, New York, USA.

·       DBT-PDF Awards (1999-2001)

Dr. Trilochan Mohapatra’s Group, NRC on Plant Biotechnology, Indian Agricultural Research Institute, Delhi.

·       NATP-Research Associateship (2001-2004)

Dr. Trilochan Mohapatra’s Group, NRCPB & Dr A.K. Singh’s Group, Division of Genetics, Indian Agricultural Research Institute, Delhi.

 

Publications : 65 Nos. ; Google citations: 1786 + and h- index is 24; i10- Index:35 ( *- Correspondence to the publications)

1. 

Parmar, R; Seth, R.; Singh, P.; Singh, G.; Kumar, S.; Sharma, R.K.* (2019) Transcriptional profiling of contrasting genotypes revealed key candidates and nucleotide variations for drought dissection in Camellia sinensis (L.) O. Kuntze. Scientific Reports 9:7487;  https://doi.org/10.1038/s41598-019-43925-w 1

2. 

Seth, R.; Bhandawat, A.; Parmar, R.; Singh, P.; Kumar, S.; Sharma, R.K.* (2019) Global Transcriptional Insights of Pollen-Pistil Interactions Commencing Self-Incompatibility and Fertilization in Tea [Camellia sinensis (L.) O. Kuntze]. Int. J. Mol. Sci. 2019, 20, 539.

3. 

Bhandawat, A.; Sharma, V.; Singh, P.; Seth, R.; Nag, A.; Kaur, J. & Sharma, R.K.* (2019). Discovery and utilization of EST-SSR marker resource for genetic diversity and population structure analyses of a subtropical bamboo, Dendrocalamu hamiltonii". Biochemical Genetics. https://doi.org/10.1007/s10528-019-09914-4

4. 

Singh, Gagandeep; Singh, G; Seth, R; Parmar, R.; Singh, P.; V. Singh, Kumar, S.; Sharma, R.K.* (2019) Functional annotation and characterization of hypothetical protein involved in blister blight tolerance in tea (Camellia sinensis (L) O. Kuntze)". J. Plant Biochem. Biotechnol.  https://doi.org/10.1007/s13562-019-00492-5

5. 

Singh, P.; Sharma, R.K.* (2019) Development of informative genic SSR markers for genetic diversity analysis of Picrorhiza kurroa. J. Plant Biochem. Biotechnol.  https://doi.org/10.1007/s13562-019-00515-1(

6.        

Singh G., Singh, Gagandeep, Singh, P., Parmar, R., Paul, N., Vashist, R., Swarnkar, M. K., Kumar, A., Singh, S., Singh, A.K., Kumar, S. & Sharma, R. K.* (2017) Molecular dissection of transcriptional reprogramming of steviol glycosides synthesis in leaf tissue during developmental phase transitions in Stevia rebaudiana Bert. Scientific Reports 7: 11835 DOI:10.1038/s41598-017-12025-y.

7.        

Singh, P., Singh, G., Bhandawat, A., Singh, G., Parmar, R., Seth, R., & Sharma, R. K.*(2017) (2017) Spatial transcriptome analysis provides insights of key gene(s) involved in steroidal saponin biosynthesis in medicinally important herb Trillium govanianum. Scientific Reports 7:45295 DOI: 10.1038/srep45295.           

8.        

Bhandawat, A., Singh, G., Seth, R., Singh, P., & Sharma, R. K.*(2017) Genome-Wide Transcriptional Profiling to Elucidate Key Candidates Involved in Bud Burst and Rattling Growth in a Subtropical Bamboo (Dendrocalamus hamiltonii). Front. Plant Sci.7:2038. doi: 10.3389/fpls.2016.02038.

9.        

Kumar, G., Gupta, K., Pathania, S., Swarnkar, M. K., Rattan, U. K., Singh, Singh, G., Sharma, R. K*., Singh, A. K. (2017) Chilling Affects Phytohormone and Post-Embryonic Development Pathways during Bud Break and Fruit Set in Apple (Malus domestica Borkh.). Scientific Reports 7:42593. doi: 10.1038/srep42593

10.     

Banerjee, A.; Mamta, D., Nag, A, Sharma, R.K., Kumar, A (2017). Modelling Probable Distribution of Podophyllum hexandrum in North-Western Himalaya. Indian Forester, 143: 1255-1259

11.     

Jayaswall, K., Mahajan, P., Singh, G., Parmar, R., Seth, R., Raina, A., Swarnkar, M.K., Singh, A.K.,  Sankar, R.,  Sharma, R.K. * (2016). Transcriptome Analysis Reveals Candidate Genes involved in Blister Blight defense in Tea (Camellia sinensis(L) Kuntze). Scientific Reports 6, 30412; doi: 10.1038/srep30412.

12.     

Singh, S., Dhyani, D., Nag, A., & Sharma, R. K (2016) Molecular characterization revealed high species level diversity among cultivated, introduced and wild roses (Rosa sp.) of western Himalayan region. Genet Resour Crop Evol 64: 515. doi:10.1007/s10722-016-0377-0

13.     

Bhandawat, A., Singh, G., Raina, A. S., Kaur, J., & Sharma, R. K.* (2016)Development of genic SSR marker resource from RNA-Seq data in Dendrocalamus latiflorus. J. Plant Biochem. Biotechnol.25:179–190. DOI 10.1007/s13562-015-0323-9. 1.(* Corresponding author)

14.     

Kumar, V., Bhandawat, A., Sharma, H., Nag, A., & Sharma, R. K.*(2016). Novel microsatellite markers identification and diversity characterization in Pteris cretica L. J. Plant Biochem. Biotechnol. 25: 104. doi:10.1007/s13562-015-0316-8.

15.     

Unamba, C.I., Nag, A. & Sharma, R.K.* £ (2015). Next Generation Sequencing technologies: The doorway to the unexplored genomics of non-model plants. Front. Plant Sci. 6:1074. doi: 10.3389/fpls.2015.01074.

16.     

Nag, A., Ahuja, P. S., & Sharma, R. K. *£ (2015) Genetic diversity of high-elevation populations of an endangered medicinal plant, AoB Plants aobpla/plu076, doi:10.1093.

17.     

Singh, P., Nag, A., Parmar, R., Ghosh, S., Bhau, B. S., & Sharma, R. K.*(2015) Genetic diversity and population structure of endangered Aquilaria malaccensis revealed potential for future conservation. Journal of Genetics 94, 697–704.

18.     

Sharma, R. K.*, Chaudhary, A., Sharma, H., Bhardwaj, P., Sharma, V., Kumar, R. , Ahuja, P. S. (2015) Identification and cross-species amplification of microsatellite markers derived from expressed sequence data of rose species. J. Plant Biochem. Biotechnol. 24: 359. doi:10.1007/s13562-014-0287-1.

19.     

Singh, P., Sharma, H., Bhau, B. S., Sharma, R. K. * (2015) Development and characterization of polymorphic microsatellites markers in endangered Aquilaria malaccensis. Conservation Genetics Resources 7: 61-63, DOI 10.1007/s12686-014-0287-4.

20.     

Bhandawat, A., Sharma, H.,  Nag, A.,  Singh, S.,  Ahuja, P. S., Sharma, R. K.*(2014) Functionally relevant novel microsatellite markers for efficient genotyping in Stevia rebaudiana Bertoni. Journal of Genetics 93: e75–e81.

21.     

Bhandawat, A., Sharma, V.,  Sharma, H.,  Sood, A.,  Sharma, R. K.*(2014) Development and cross transferability of functionally relevant microsatellite markers in Dendrocalamus latiflorus and related bamboo species. Journal of Genetics 93, e48–e55.

22.     

Kumar, N. Taneja, Sharma, R. K.,  Sharma, H.,  Ramamurthy, T.,  Sharma, M.  (2014) Molecular characterization of Shiga-toxigenic Escherichia coli isolated from diverse sources from India by multiple locus variable number tandem repeat analysis (MLVA). Epidemiology and Infection, 142(12):2572-82. doi: 10.1017/S0950268814000065.

23.     

Bajpai, P. K., Warghat, A. R., Sharma, R. K., Yadav, A.,  Thakur, A. K., Srivastava, R.B. & Stobdan, T. (2014) Structure and genetic diversity of natural populations of Morus alba in the trans-Himalayan Ladakh region. Biochemical Genetics 52:137–152.

24.     

Bhardwaj, P., Sharma, R. K.,  Kumar, R.,  Sharma, H., Ahuja, P. S.  (2014) SSR Marker Based DNA Fingerprinting and Diversity Assessment in Superior Tea Germplasm Cultivated in Western Himalaya. Proc Indian Natn Sci Acad 80 (1): 157-162

25.     

Bhardwaj, P.,  Kumar, R.,  Sharma, H. , Tewari, R.,  Ahuja, P. S. & Sharma, R. K.*(2013) Development and utilization of genomic and genic microsatellite markers in Assam tea (Camellia assamica spp. assamica) and related Camellia species. Plant Breeding 132: 748–763.

26.     

Nag, A., Bhardwaj, P., Ahuja, P. S. & Sharma, R. K.* (2013) Identification and Characterisation of 20 novel unigene derived microsatellite markers in Podophyllum hexandrum. Journal of Genetics 92: e4-e7.

27.     

Nag, A.,  Gupta, P. , Sharma, V.,  Sood, A.,  Ahuja, P. S. & Sharma, R. K.* (2013). AFLP and RAPD based genetic diversity assessment of industrially important reed bamboo (Ochlandra travancorica Benth). J. Plant Biochem & Biotechnol: 22 1:144-149.

28.     

Singh, S.,  Sud, R. K.,  Gulati, A.,  Joshi, R., Yadav, A. K., Sharma, R. K. (2013) Germplasm appraisal of western Himalayan tea: a breeding strategy for yield and quality improvement. Genetic Resources & Crop Evolution  60:1501–1513.

29.     

Raina, S.N., Ahuja, P. S.,  Sharma, R. K.et al (2012). Genetic structure and diversity of India Hybrid Tea. Genetic Resources & Crop Evolution 59:1527–1541 doi:10.1007/s10722-011-9782-6.

30.     

Korekar, G.,  Sharma, R. K., Kumar, R., Meenu, Bisht, N.C.,  Srivastava, R. B.,  Ahuja, P. S. &  Stobdan, T.  (2012). Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae). Biotechnol Lett, 34:973–978.

31.     

SivaRaju, K., Sharma, R. K. & Murthy, T.G.K. (2012). Genetic and Evolutionary relationship among Nicotina species as elucidated by AFLP. The Journal of Agricultural Sciences 7:135-144.

32.     

Sharma, H., Kumar, R., Sharma, V., Kumar, V., Bhardwaj, P., Ahuja, P. S. & Sharma, R. K.*(2011) Identification and cross-species transferability of 112 Novel unigene-derived microsatellite markers in tea (Camellia sinensis). Am. J. Bot. 98:e133-e138. 1.

(£ Corresponding author)

33.     

Mehta, R., Sharma, V., Sood, A.,  Sharma, M. & Sharma, R. K.*(2011) Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro derived plantlets of Bambusa nutans Wall. using AFLP markers. Eur J Forest Res 130:729–736.

34.     

Mishra, A.#,  Taneja, N. #,  Sharma, R. K.#, Kumar, R. #,  Sharma, N.C. # &  Sharma, M. # (2011) Amplified fragment length polymorphism of clinical and environmental Vibrio cholerae from a freshwater environment in a cholera-endemic area, India. BMC Infectious Diseases 11:249. ( #equal contribution)

35.     

Swapna, M., Sivaraju, K., Sharma, R. K.*, Singh, N. K. & T. Mohapatra (2011). Single-Strand Conformational Polymorphism of EST-SSRs: A Potential Tool for Diversity Analysis and Varietal Identification in Sugarcane. Plant Mol Biol Rep 29:505–513.

36.     

Nadha Kaur, H., Kumar, R., Sharma, R. K*., Anand, M. & Sood, A., (2011) Evaluation of clonal fidelity of in vitro raised plants of Guadua angustifolia Kunth using DNA-based markers. J Med Plants Research (JMPR) 5:5636-5641.

37.     

Kamunya, S. M., Wachira, F. N., Pathak, R. S., Korir, R., Sharma, V., Kumar, R., Bhardwaj, P., Chalo, R., Ahuja P.S. & Sharma, R. K.* (2010) Genomic mapping and testing for quantitative trait loci in tea (Camellia sinensis (L.) O. Kuntze). Tree Genetics & Genomes 6:915–929.

38.     

Sharma, R. K.*, Negi, M. S., Sharma, S., Bhardwaj, P., Kumar, R., Bhattachrya, E., Tripathi, S. B., Vijayan, D., Baruah, A. R., Das, S. C., Bera, B.,  Rajkumar, R. , Thomas, J.,  Sud, R. K., Muraleedharan, N.,  Hazarika, M.,  Lakshmikumaran, M.,  Raina, S. N. &  P. S. Ahuja (2010). AFLP-based genetic diversity assessment of commercially important tea germplasm in India. Biochemical Genetics 48:549–564.

39.     

Kamunya, S. M., Wachira, F. N., Owuor, P. O., Pathak, R. S., Wanyoko, J. K., Sharma, R. K., & Muoki, R. C.  (2010). Quantitative genetic parameters for yield, drought tolerance and some quality traits in tea (Camellia sinensis L.) O. Kuntze. Research J of Agri Sci 1(2): 53-65.

40.     

Singh, R. K., Sharma, R. K.,  Singh, A. K.,  Tewari, S. P. & Mohapatra, T. (2010). Cost effective method for detection of admixture in traditional Basmati rice using mapped sequence tagged microsatellite markers. Natl Acad Sci Lett 33: 341-346.

41.     

Sharma, R.K*., Bhardwaj, P., Negi, R., Mohapatra, T., & Ahuja, P.S. (2009). Identification, characterization and utilization of unigene derived microsatellite markers in tea (Camellia sinensis, L). BMC Plant Biology 9:53. 1.     

42.     

Sharma, V., Bhardwaj, P., Kumar, R., Sharma, R. K.*, Sood, A., & Ahuja, P. S. (2009). Identification and cross-species amplification of EST derived SSR markers in different bamboo species. Conservation Genetics 10:721–724. 1.         

43.     

Kaul, K., Karthigeyan, S., Dhyani, D., Kaur, N., Sharma, R. K., & Ahuja, P. S. (2009). Morphological and molecular analyses of Rosa damascena x R. bourboniana interspecific hybrids. Scientia Horticulturae 122 (2009) 258–263.

44.     

Mohapatra, K. P., Sehgal, R. N., Sharma, R. K., & Mohapatra, T. (2009). Genetic analysis and conservation of endangered medicinal tree species Taxus wallichiana in the Himalayan region. New Forests 37:109–121.

45.     

Kamunya, S. M., Wachira, F. N., Pathak, R. S., Muoki, R. C., Wanyoko, J. K., Ronno, W. K., & Sharma, R. K. (2009). Quantitative genetic parameters in te

 A.                 International Fellows

1.                   Mr. Tony, TWAS Fellow Ph.D candidate (TRFK, Kenya)

B.                  Post-Doctoral Fellows/ Research Associates  

1.                   Dr Praveen Dhayni, Research Associate

2.                   Dr. Shruti Choudhary, Research Associate

C.                  Ph.D. Scholars

1.                   Mr. Pradeep Singh, Junior Research Fellow, Ph.D candidate

2.                   Ms. Rajni Parmar, Senior Research Fellow, Ph.D candidate

3.                   Mr. Romit Seth, Junior Research Fellow, Ph.D candidate

4.                   Mr. Gopal Singh, Senior Research Fellow, Ph.D candidate

5.                   Ms. Priyanka Dhaka, Junior Research Fellow, Ph.D candidate

6.                   Ms. Poonam Pal, Junior Research Fellow, Ph.D candidate

D.                 Research Fellows

1.                   Mr. Praveen Thakur, Project Assistant

2.                   Mr. Balraj Sharma¸ Project Assistant

3.                   Ms. Mamta, Project Assistant

4.                   Ms. Amna, Project Assistant

5.                   Ms. Ashlesha, Project JRF

6.                   Ms. Shivanti Negi, Project Assistant

7.                   Ms. Aprajita Sood, Project Assistant

8.                   Mr. Rashmi Ranjan Sahu¸ Project Assistant

9.                   Ms. Shikha Sharma, Project JRF

10.               Mr. Rohan Kale, Project Assistant

11.               Ms. Shivani Rana, Project Assistant

12.               Mr. Shivanshu Garg, Project Assistant

13.               Ms. Aprajita Sood, Project Assistant

Former Members

1.     Dr. Samson M. Kamunya, CSIR-TWAS Fellow, Ph.D Candidate (Currently: Principal Breeder, TRFK, Kenya)

2.               Mr. Unamba Chibuikem, TWAS Fellow Ph.D candidate (Nigeria) (2015, 2016)

3.                   Miss Eneni M.I. Roberts, JNCASR-CICS Research Fellow (Nigeria)

4.                   Dr. Pankaj Bhardwaj, Senior Research Fellow, Ph.D Candidate (Currently :Assistant Professor, Central Univ, Bhatinda)

5.                   Dr. Rahul Kumar,  Senior Research Fellow, Ph.D Candidate (Currently:Assistant Professor, DAV Univ, Jallandhar

6.                   Dr. Vikas Sharma,  Senior Research Fellow, Ph.D Candidate (Currently:D.S. Kothari Post-Doc Fellow)

7.                   Dr. Akshay Nag, Research Associate, Ph.D. candidate (Currently: National PDF, NRC on Plant Biotechnology, IARI Campus, New Delhi, India)

8.                   Dr. Himanshu Sharma, Senior Research Fellow, Ph.D Candidate (Currently: National PDF, NABI, Chandigarh)

9.                   Mr. Kuldip Jayaswal, Junior Research Fellow, Ph.D candidate (Currently: ARS Scientist, NARM, Hyderabad

10.               Dr Sandeep Rawat, DST Young Scientist (Currently: Scientist C, G.B.P.I.H.E.D, Sikkim

11.               Dr. Abhishek Bhandawat, Ph.D candidate (Currently: National PDF, NABI, Chandigarh)

12.               Mr. Shivshankar Mittal, Project Assistant -II

13.               Ms. Priyanka Gupta, Research Fellow

14.               Ms. Rinu Negi, Research Fellow

15.               Mr. Vishal Kumar, Junior Research Fellow, Ph.D Candidate

16.               Mr Yogendra, Junior Research Fellow

17.               Ms. Navgeet, Junior Research Fellow

18.               Mr. Vishal Tripathi, Junior Research Fellow

19.               Ms. Radhika Vashisht, Research Fellow

20.               Mr. Anil Chaudhary, Project Fellow

21.               Ms. Deepika Sharma, Project Fellow

22.               Ms. Ragini Sharma, Project Fellow

23.               Ms. Vandana, Project Fellow

24.               Ms Palak, Project Assistant-II

25.               Ms. Himakshi Thakur, Senior Research Fellow, Ph.D candidate

26.               Mr. Gagandeep Singh, Senior Research Fellow, Ph.D candidate

27.               Ms. Urvashi dave, Project Assistant

28.               Mr. Vinod Yadav, Junior Research Fellow

29.               Mr. Vishal Koundal, Project Assistant

30.               Ms Payal Kotvi, Project Assistant

31.               Ms .Anu Sharma, Project Assistant

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