Virus-free chrysanthemum

TAV along with CMV and CVB infecting chrysanthemum cv. Regol Time either alone or in mixed conditions were eradicated from the plant by in vitro techniques combined with meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by bioassay, ELISA and RT-PCR techniques. Tissue cultured CMV, CVB and TAV-free chrysanthemum plants grown in the field showed significant increase in plant height, length of spray or branch, number of spray/plant, number of flower/spray, number of flower/plant, diameter of flower and duration of flowering in comparison to diseased plants.

Alstroemeria

CMV free in vitro alstroemeria plants were produced in the virus-infected hybrid cv Serena using meristem tip culture and thermotherapy. Meristem tips (0.3-0.4 mm size) from virus-infected plants were grown in MS medium supplemented with 3 % sucrose, BAP (2 mg/l) and NAA (1 mg/l) with pH 5.7. The plants grown from meristem tips were transferred in MS medium supplemented with BAP (1 mg/l); NAA (0.1 mg/l) and sucrose 1.5 % for rooting and incubated at 37-38°C for 35-40 days. Plants developed with roots (72 %) were found to be CMV-free by DAS-ELISA using CMV-specific antibodies.

		Production of CVB-free chrysanthemum cv. Regol Time:
Cucumber mosaic virus- free alstroemeria cv Serena produced in vitro. (A) Meristem tip in culture, (B) Plantlets initiation from meristem tip and (C) proliferation stage
			Production of CVB-free chrysanthemum cv. Regol Time: