EXPRESSION OF Potentilla Cu-Zn SOD GENE IN Arabidopsis

The aim is to  study the effect of Cu-ZnSOD gene in transgenic Arabidopsis. This study will help in understanding the essential role of Cu-ZnSOD in adaptive responses of plant cells under environmental stresses.

Preparation of the Cu-Zn SOD construct

The full length Cu-ZnSOD gene (456bp) was cloned in a cloning vector. The desired restriction sites were incorporated into the tails of the primers which was used for amplification. The confirmed positives were sequenced for error free clones. The cloned SOD gene was isolated from such clones and again sub-cloned into the expression vector (pCAMBIA). This construct was transformed into Arabidopsis through vacuum infiltration via Agrobacterium. The positives were screened on selective medium plates (Fig. 4.21). From the plates the positives were transferred on to pots. These transformants were further confirmed by using the genomic DNA as a template from the leaves of these plants (Fig.4.22). The gene behaviour and its expression studies are in progress.