Method
for inducing improved seed germination in Podophyllum
hexandrum Royle (6449899)
Abstract
The present invention describes a method for inducing improved seed
germination in a high altitude medicinal plant, Podophyllum
hexandrum Royle through hot
water treatment at 40.degree. C.-100.degree. C. for 30-120 seconds.
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Inventors: |
Kharkwal; Amit Chandra (Palampur, IN),
Prakash; Om (Palampur, IN), Bhattacharya; Amita (Palampur, IN),
Nagar; Pramod Kumar (Palampur, IN), Ahuja;
Paramvir Singh (Palampur,
IN) |
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Assignee: |
Council of Scientific and Industrial Research ( |
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Appl. No.: |
09/800,672 |
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Filed: |
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Claims
What is claimed is:
1. A method for inducing improved seed germination at high altitudes in an
endangered medicinal plant, Podophyllum
hexandrum Royle, said
method comprising the steps of: (a) collecting mature pods of P. hexandrum Royle, (b) separating
the seeds from the pulp, (c) disinfecting the seeds with 0.1% carbendazim and 1% streptomycin sulphate
for 15 minutes, (d) treating the fresh seeds with hot water ranging from
40-100.degree. C. for varied time intervals ranging from 30-120 seconds, (e)
sowing the hot water treated seeds in pots containing sand:soil:farm yard manure in the ratio 2:1:1 at a depth
of 1/2 inch, (f) maintaining the sown seeds in the pots at a minimum of
30.degree. C. during day and at least 20.degree. C. during night and relative humidity of 60-70%, and (g) sprinkling
water at least once after every two days.
2. A method as claimed in claim 1, wherein the method
is repeatable for two successive years.
3. A method as claimed in claim 1 wherein the seeds
are separated from their pulp immediately after collection.
4. A method as claimed in claim 1 wherein the seeds are sown in 8" pots
containing the potting mix in poly-tunnel.
5. A method as claimed in claim 1 wherein the growth conditions for seed
germination and seedling establishment and true leaf emergence are under
green-house.
6. A method as claimed in claim 1 wherein the seeds after sowing are allowed to
mature under green- or poly-house conditions in pots containing the potting mix
in poly-tunnel.
7. A method for inducing improved seed germination at high altitudes in an
endangered medicinal plant, Podophyllum
hexandrum Royle, said
method comprising the steps of: (a) collecting mature pods of P. hexandrum Royle, (b) separating
the seeds from the pulp, (c) disinfecting the seeds with 0.1% carbendazim and 1% streptomycin sulphate
for 15 minutes, (d) treating the fresh seeds with hot water ranging from 40-100.degree.
C. for varied time intervals ranging from 30-120
seconds, (e) sowing the hot water treated seeds in container or beds containing
a potting mix of sand, soil and farm yard manure at a depth of from 1/2 to 2
inches, (f) maintaining the sown seeds at a minimum of 30.degree. C. during day and at least 20.degree. C.
during night and relative humidity of 60-70%, and (g) sprinkling water after
every one to three days.
8. A method as claimed in claim 7, wherein the method
is repeatable for two successive years.
9. A method as claimed in claim 7, wherein the seeds
are separated from their pulp immediately after collection.
10. A method as claimed in claim 7, wherein the potting mix contains sand:soil:farm yard manure in the
ratio 2:1:1.
11. A method as claimed in claim 7, wherein the hot water treated seeds are
sown in the potting mix at a depth of 1/2 inch.
12. A method as claimed in claim 7, wherein the seeds are sown in 8" pots
containing the potting mix in poly-tunnel.
13. A method as claimed in claim 7, wherein the growth conditions for seed
germination and seedling establishment and true leaf emergence are under
green-house.
14. A method as claimed in claim 7, wherein the seeds after sowing are allowed
to mature under green- or poly-house conditions in pots containing the potting
mix in poly-tunnel.
15. A method as claimed in claim 7, wherein the water
is sprinkled at least once after every two days.