Developed a microanalytical method to determine and quantify resin and podophyllotoxin in the raw material. Podophyllotoxin present in plant or cell culture can be determined with minimum quantity (2 mg) with consistent results
Podophyllotoxin (%) content using microanalytical method
|
Quantity
of plant material (mg) |
Podophyllotoxin (%) |
|
40 |
4.46 |
|
30 |
4.29 |
|
5 |
4.36 |
|
2 |
4.30 |
Also, another method was
standardized for determination of resin in raw material and podophyllotoxin up to 100 mg of raw material with
almost consistent results
Resin and podophyllotoxin content using microanalytical
method
|
Quantity
of plant material (mg) |
Resin
(%) |
Podophyllotoxin (%) |
|
400 |
3.4 |
3.2 |
|
300 |
4.4 |
3.1 |
|
200 |
4.9 |
2.8 |
|
100 |
5.0 |
2.7 |
Methanolic extract of P.hexandrum on repeated chromatographic separation yielded two pure compounds. One was characterized as podophyllotoxin on the basis of spectral analysis and second is being elucidated.