FULL-LENGTH cDNAs CLONED FROM HIMALAYAN FLORA
The total genes that were isolated and cloned from the Himalayan flora at IHBT is presented (Table 4.4). This work was carried out under various in-house and sponsored projects.
Table 4.4 Full lengths cDNAs cloned from Himalayas flora
|
S.No |
Gene Name |
Source |
Reference ( NCBI Acc No.) |
Project |
|
1 |
QM gene |
Caragana jubata |
AY641430 |
GAP-0049 |
|
2 |
Lipoxygenase |
Caragana jubata |
in process |
GAP-0049 |
|
3 |
Succinyl CoA ligase |
Caragana jubata |
in process |
GAP-0049 |
|
4 |
Myc-like anthocyanin regulatory protein (basic helix loop helix)) |
Caragana jubata |
DQ458766 |
GAP-0049 |
|
5 |
Copper/zinc superoxide dismutase |
Potentilla atrosanguinea |
in process |
GAP-0049 |
|
6 |
Basic helix-loop-helix |
Rheum emodi |
DQ078120 |
GAP-0049 |
|
7 |
Mitogen activated protein kinase 1 |
Rheum emodi |
DQ078119 |
GAP-0049 |
|
8 |
Mitogen activated protein kinase 2 |
Rheum emodi |
DQ241823 |
GAP-0049 |
|
9 |
Manganese superoxide dismutase |
Rheum emodi |
in process |
GAP-0049 |
|
10 |
Ascorbate peroxidase |
Rheum emodi |
DQ078123 |
GAP-0049 |
|
11 |
Copper/zinc superoxide dismutase |
Rheum emodi |
DQ078121 |
GAP-0049 |
|
12 |
Calmodulin |
Rheum emodi |
in process |
GAP-0049 |
|
13 |
Histone H3 |
Rheum emodi |
DQ078122 |
GAP-0049 |
|
14 |
60S ribosomal protein L35 |
Rheum emodi |
DQ126007 |
GAP-0049 |
|
15 |
Glutathione reductase |
Rheum emodi |
in process |
GAP-0049 |
|
16 |
Monodehydroascorbate reductase |
Rheum emodi |
in process |
GAP-0049 |
|
17 |
Caffeine synthase |
Camellia sinensis |
AY907710 |
NMITLI |
|
18 |
4- Coumaroyl CoA ligase |
Camellia sinensis |
DQ194356 |
NMITLI |
|
19 |
Chalcone isomerase |
Camellia sinensis |
DQ120521 |
NMITLI |
|
20 |
Flavonoid 3´5´dihydroxylase |
Camellia sinensis |
DQ194358 |
NMITLI |
|
21 |
Phenylalanine ammonia lyase |
Camellia sinensis |
AY694188 |
NMITLI |
|
22 |
Cinnamate 4- hydroxylase |
Camellia sinensis |
AY641731 |
NMITLI |
|
23 |
Chalcone synthase |
Camellia sinensis |
AY656677 |
NMITLI |
|
24 |
Flavonoid 3’ hydroxylase |
Camellia sinensis |
AY641730 |
NMITLI |
|
25 |
Dihydroflavonol reductase |
Camellia sinensis |
AY648027 |
NMITLI |
|
26 |
Anthocyanin reductase |
Camellia sinensis |
AY641729 |
NMITLI |
|
27 |
Manganese superoxide dismutase |
Camellia sinensis |
AY641734 |
NMITLI |
|
28 |
Anthocyanin synthase |
Camellia sinensis |
AY830416 |
NMITLI |
|
29 |
Polyphenol oxidase |
Camellia sinensis |
AY659975 |
CMM-0004 |
|
30 |
Metallothionein-like protein |
Camellia sinensis |
DQ444292 |
GAP-0041 |
|
31 |
Metallothionein 1 |
Camellia sinensis |
DQ442270 |
GAP-0041 |
|
32 |
Metallothionein 2 |
Camellia sinensis |
DQ442271 |
GAP-0041 |
|
33 |
Ascorbate peroxidase |
Camellia sinensis |
DQ442272 |
GAP-0041 |
|
34 |
Thaumatin-like protein |
Camellia sinensis |
DQ444296 |
GAP-0041 |
|
35 |
Phragmoplastin |
Camellia sinensis |
DQ444295 |
GAP-0041 |
|
36 |
cdk Regulatory subunit |
Camellia sinensis |
DQ444293 |
GAP-0041 |
|
37 |
Alpha tubulin |
Camellia sinensis |
DQ444294 |
GAP-0041 |
|
38 |
ATPase |
Camellia sinensis |
DQ461974 |
GAP-0041 |
|
39 |
QM gene |
Camellia sinensis |
AY641733 |
GAP-0041 |
|
40 |
AP7 (hypothetical protein) |
Camellia sinensis |
In process |
GAP-0041 |
|
41 |
AP37 (hypothetical protein) |
Camellia sinensis |
In process |
GAP-0041 |
|
42 |
Acetoacetyl CoA thiolase |
Picrorhiza kurrooa |
DQ347964 |
COR-0002 |
|
43 |
1-Deoxy-D-xylulose-5- phosphate reductoisomerase |
Picrorhiza kurrooa |
DQ347963 |
COR-0002 |
|
44 |
3-Hydroxy-3-methylglutaryl-CoA reductase |
Picrorhiza kurrooa |
DQ347962 |
COR-0002 |
|
45 |
Geranyl dihosphate synthase |
Picrorhiza kurrooa |
AY866498 |
COR-0002 |
|
46 |
Thiamine biosynthetic gene |
Picrorhiza kurrooa |
AY866497 |
COR-0002 |
|
47 |
Acetoacetyl-CoA thiolase |
Arnebia euchroma |
in process |
CMM-0002 |
|
48 |
HMG Co-A Reductase |
Arnebia euchroma |
in process |
CMM-0002 |
|
49 |
Geranyl diphosphate synthase |
Arnebia euchroma |
in process |
CMM-0002 |
|
50 |
Isopentenyl pyrophosphate isomerase |
Arnebia euchroma |
in process |
CMM -0002 |
|
51 |
PHB geranyl transferase ( P- hydroxyl benzoic) |
Arnebia euchroma |
in process |
CMM-0002 |
|
52 |
Phenylalanine ammonia lyase |
Arnebia euchroma |
in process |
CMM-0002 |
|
53 |
Cinnamate 4- hydroxylase (C4H) |
Arnebia euchroma |
in process |
CMM-0002 |
|
54 |
LEDI-2 (Dark inducible) |
Arnebia euchroma |
in process |
CMM-0002 |
|
55 |
4-Diphosphocytidyl-2-C-methyl-D-Erythritol Synthase (DMES) |
Stevia rebaudiana |
DQ269452 |
CMM-0014 |
|
56 |
4-Diphosphocytidyl-2-C-methyl-D-erythritol kinase (DMEK) |
Stevia rebaudiana |
DQ269453 |
CMM-0014 |
|
57 |
2-C-methyl-D-erythritol -2,4-cyclodiphosphosphate synthase (DMEC) |
Stevia rebaudiana |
DQ631427 |
CMM-0014 |
|
58 |
IPP/DMAPP Synthase (IDS) |
Stevia rebaudiana |
DQ269451 |
CMM-0014 |
|
59 |
Geranyl-geranyl diphosphate synthase (GGDPS) |
Stevia rebaudiana |
CMM-0014 |
|
|
59 |
Kaurenoic acid 13-Hydroxylase (KAH) |
Stevia rebaudiana |
DQ398871 |
CMM-0014 |
|
60 |
NADPH cytochrome P450 reductase
|
Stevia rebaudiana |
DQ269454 |
CMM-0014 |
|
61 |
Malate dehydrogenase (MDH) |
Stevia rebaudiana |
DQ269456 |
CMM-0014 |
|
62 |
Aquaporin |
Stevia rebaudiana |
DQ269455 |
CMM-0014 |
|
63 |
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (rbcL) |
Stevia rebaudiana |
CMM-0014 |
|
|
64 |
ABC Transporter family gene |
Stevia rebaudiana |
DQ232762 |
CMM-0014 |
|
65 |
P450 monooxygenase |
Stevia rebaudiana |
DQ232763 |
CMM-0014 |
|
66 |
Glutamine synthetase |
Stevia rebaudiana |
CMM-0014 |
|
|
67 |
Photosystem II D1 protein |
Stevia rebaudiana |
CMM-0014 |
|
|
68 |
Fructose bisphosphate aldolase |
Stevia rebaudiana |
DQ437512 |
CMM-0014 |
|
69 |
Myc-like regulatory protein (basic helix loop helix) |
Stevia rebaudiana |
in process |
CMM-0014 |
|
70 |
TTG1 like WD40 repeat transcription factor |
Stevia rebaudiana |
in process |
CMM-0014 |
|
71 |
AN11 like WD40 repeat transcription factor |
Stevia rebaudiana |
in process |
CMM-0014 |
GAP-0041: Characterization and improvement of tea through biotechnological approach (Sponsored by Department of Biotechnology, New Delhi).
GAP-0049: Novel products from western Himalayas: Antioxidant formulation: Cloning of temperature related cDNA and development of microbial formulations (Sponsored by Department of Biotechnology, New Delhi).
CMM-0002: Developing cell and tissue engineering for colours and dyes (Sponsored by CSIR, New Delhi).
CMM-0004: Designing plants and animals as Bioreactors for proteins and other products (Sponsored by CSIR, New Delhi).
CMM-0014: Positioning Indian nutraceuticals and nutrigenomics in a global platform (Sponsored by CSIR, New Delhi).
COR-0002: Development of medicinal plant chemotypes for enhanced marker and value added compounds (Sponsored by CSIR, New Delhi).
NIMITLI: Niche pathway engineering in tea. (Sponsored by CSIR, New Delhi).